Abstract
To reveal how DNA/cationic carrier complexes physicochemically behave in cells, dual color fluorescence fluctuation spectroscopy (dual color FFS) is a promising tool. FFS monitors the fluorescence fluctuations caused by the diffusion of fluorescently labeled molecules through the excitation volume of a microscope. Dual color FFS answers the question whether the DNA and the cationic carrier move together (i.e. when they are associated) or separately (i.e. when they are dissociated) through the excitation volume.
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