Abstract
This chapter focuses on the synthesis of covalent enzyme-coenzyme complexes of liver alcohol dehydrogenase and nicotinamide adenine dinucleotide (NAD). The problems of retention and regeneration are solved by two methods used for the preparation of complexes between alcohol dehydrogenase and NAD. The first method consists of the immobilization of the reduced NAD analog, N 6 -[ N -(6-aminohexyl)carbamoylmethyl]NADH, and the enzyme, liver alcohol dehydrogenase, to a support such activated agarose. The second method provides more alternatives for regeneration. The broad substrate specificity of liver alcohol dehydrogenase for redox reactions makes it a versatile enzyme for use in organic reactions. The complex of liver alcohol dehydrogenase-NADH and agarose is stable and easy to prepare and might be useful in the production of various ketones, aldehydes, and alcohols. The soluble complex of liver alcohol dehydrogenase and NAD also function in enzyme reactions with other NAD-dependent dehydrogenases where both enzymes share a common coenzyme, though it is covalently bound to liver alcohol dehydrogenase.
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