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Abstract
Objective: We find that the biogenesis of mitochondria is response to the early stage of cholestasis with internal pathway. The regulation pathogenesis of the liver cell apoptosis in this early stage in cholestatic liver injury is not clear. Methods: A rat model of cholestasis was established by bile duct ligation (BDL), with simultaneous creation of the sham group receiving laparotomy without BDL. Liver LC3B1, LC3B2, Beclin-1, caspase 9 protein expression was analyzed by western blotting. The ATF6, IRE1, BIP RNA level was measured by realtime polymerase chain reaction. Results: The liver LC3B-2 and LC3B2/LC3B1 ratio at 6-72 hours with caspase 9 activity and Beclin-1 levels at 72 hours significant upregulated. The ATF6, IRE1, BIP significantly decreased at 24- 72 hours. Conclusions: Our results indicate that regulation of the liver biogenesis is within a few hours after complete bile duct obstruction. Early murine cholestatic liver injury is via autophagy pathway.
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