749 DECREASE OR LOSS OF PTEN EXPRESSION IS FREQUENT IN PENILE SQUAMOUS CELL CARCINOMAS

Abstract

You have accessJournal of UrologyPenis/Testis/Urethra: Benign & Malignant Disease II1 Apr 2012749 DECREASE OR LOSS OF PTEN EXPRESSION IS FREQUENT IN PENILE SQUAMOUS CELL CARCINOMAS Alcides Chaux, Antonio L. Cubilla, Jessica Hicks, Kristen Lecksell, and George Netto Alcides ChauxAlcides Chaux Baltimore, MD More articles by this author , Antonio L. CubillaAntonio L. Cubilla Asunción, Paraguay More articles by this author , Jessica HicksJessica Hicks Baltimore, MD More articles by this author , Kristen LecksellKristen Lecksell Baltimore, MD More articles by this author , and George NettoGeorge Netto Baltimore, MD More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.835AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Loss of PTEN tumor suppressor gene expression is a common genetic alteration in several types of malignancies. Data on PTEN status in penile squamous cell carcinomas (SCC) is scant. Herein, we evaluate PTEN expression in a large series of penile tumors. METHODS One-hundred and twelve cases of penile SCC were used to build 4 tissue microarrays (TMA). Each tumor was sampled 312 times. TMA spots were scanned using the APERIO system and uploaded to the TMAJ platform (http://tmaj.pathology.jhmi.edu). Cytoplasmic PTEN expression was assessed by immunohistochemistry, using a previously described protocol (Am J Surg Pathol 2011;35:1549). A case was categorized as showing PTEN loss when complete PTEN loss of expression was encountered in all TMA spots. A case was classified as retained PTEN expression when all TMA spots expressed PTEN in all cells. All remaining cases were classified as decreased PTEN expression. RESULTS PTEN expression was retained in 13 cases (12%), decreased in 84 cases (75%), and lost in 15 cases (13%). Distribution of histological subtypes were similar in cases with retained and decreased PTEN expression (P = .77). However, cases with PTEN loss showed a higher proportion of warty/basaloid carcinomas (80%) than keratinizing carcinomas (20%). This difference was significant (P = .02). Histological grade was not associated with PTEN expression (P = .20). CONCLUSIONS The majority (88%) of penile SCC showed decrease or loss of PTEN expression. PTEN loss was observed more frequently in warty/basaloid carcinomas, independent of histological grade. Additional studies are required to further characterize this association and to explore any potential relationship between PTEN loss and HPV infection. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e307 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information Alcides Chaux Baltimore, MD More articles by this author Antonio L. Cubilla Asunción, Paraguay More articles by this author Jessica Hicks Baltimore, MD More articles by this author Kristen Lecksell Baltimore, MD More articles by this author George Netto Baltimore, MD More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...