744 Genome-Wide Association of Composition and Diversity of the Intestinal Bacterial Phyla in Healthy First Degree Relatives (FDR) of Crohn's Disease (CD) Subjects

Abstract

Figure 1: Sepp1-/enteroids display an increase in stem cell characteristics and increased ROS production, proliferation, and decreased survival in response to hydrogen peroxide administration. (A) Relative Sepp1 mRNA expression. (B) Enteroid growth properties. (C) ROS quantification after 2 hours of treatment (left). Representative images (100x magnification, right). (D) Proliferation quantification as determined by EdU+ cells per crypt area. (100x magnification, right). (E) Survival curves for WT (red), Sepp1 (blue), and Sepp1 (black) enteroids after daily treatment with 400 μM hydrogen peroxide. *P<0.05, **P<0.01, ***P<0.001.