731 Generation of a laser capture microdissection and RNAseq-based human anagen hair follicle transcriptome atlas

Abstract

Characterizing the transcriptome of defined hair follicle (HF) compartments and cell populations is a fundamental hair research challenge, which has already been mastered for murine HFs (www.hair-gel.net). Yet, a comprehensive atlas of the human HF transcriptome remains to be generated. As a key step into this direction, we have used laser capture microdissection coupled with RNA sequencing to reveal the gene expression profiles of 8 selected compartments of human anagen VI HFs. Principal component analysis (PCA) identified a high degree of similarity of the distinct transcriptional profile of each examined compartment between biological replicates and a clear separation of the HF compartments.