Abstract
Methods: The forearm cortical skin stratum corneum samples were obtained from 11 healthy subjects by a non-invasive method that cyanoacrylate adhesive method after signing the informed consent form; 4 blood samples were obtained by intravenous blood collection after written informed consent. Identification of ceramide in skin and blood samples is used by UltimateTM3000 high performance liquid chromatography coupled with Q - Exactive TM quadrupole - electrostatic field orbital trap. Data was analyzed using full-flow lipid analysis software (Lipid Search) after collected. Results: Experiments were performed on 11 skin samples and 4 blood samples for ceramide characterization. Peaks can be seen in all skin samples with response intensities between 105 and 107, with peaks in some blood sample. Positive ions and negative ions were collected respectively for the samples. Except for the target ceramides reported in the literature, more than 300 other lipids were detected under positive ion conditions and more than 30 were detected under negative ion conditions, the results show that the ceramides in the skin samples are mainly long-chain ceramides and the blood samples are mainly short-chain ceramides. Conclusion: In this experiment, the qualitative analysis of ceramide in the samples can be carried out quickly and easily under the premise of maximizing the noninvasive and the differences in the distribution of ceramides in different tissues of the human body are demonstrated, indicating the importance of ceramide for skin barrier and the application prospect in the cosmetics industry.
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