725 PROSTATIC DISEASES IN THE ELDERLY MEN: GLANDULAR MICROENVIRONMENT SIGNALING OF IGFR-1, STEROID HORMONE RECEPTORS, MATRIX METALLOPROTEINASES AND DYSTROGLYCANS

Abstract

You have accessJournal of UrologyProstate Cancer: Basic Research1 Apr 2011725 PROSTATIC DISEASES IN THE ELDERLY MEN: GLANDULAR MICROENVIRONMENT SIGNALING OF IGFR-1, STEROID HORMONE RECEPTORS, MATRIX METALLOPROTEINASES AND DYSTROGLYCANS Valéria Quitete, Amanda Hetzl, Wagner Fávaro, Ubirajara Ferreira, and Athanase Billis Valéria QuiteteValéria Quitete Campinas, Brazil More articles by this author , Amanda HetzlAmanda Hetzl Campinas, Brazil More articles by this author , Wagner FávaroWagner Fávaro Campinas, Brazil More articles by this author , Ubirajara FerreiraUbirajara Ferreira Campinas, Brazil More articles by this author , and Athanase BillisAthanase Billis Campinas, Brazil More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.1694AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Senescence is a determining factor for the occurrence of morphological changes in the prostate. Thus, the objective of this work was to characterize and correlate the α and β-dystroglycans (αDG, βDG), androgen receptor (AR), α and β-estrogen receptors (αER, βER), matrix metalloproteinases 2 and 9 (MMP-2, MMP-9) and insulin-like growth factor receptor (IGFR-1) reactivities in both stroma and epithelium compartments of elderly men with Benign Prostatic Hyperplasia (BPH), Prostatic Intraepithelial Neoplasia (HGPIN) and Prostatic Cancer (PC). METHODS Sixty prostatic peripheral zone samples from 60–90 year old patients were divided into 4 groups: The Standard Group (no lesions); The HGPIN Group; The PC and The BPH Group and analyzed by means of immunohistochemistry and Western Blotting analyses. RESULTS The results showed increased IGFR-1, MMP-2 and MMP-9 protein levels in the PC and PIN groups in relation to other groups. Decreased αDG and βDG protein levels were verified in the PC and PIN groups in relation to the BPH and Standard groups. Intensified AR immunoreactivity was verified in the epithelial compartment in all studied groups. αER immunoreactivity was more intense in the epithelial compartment in the PC and PIN groups than in the other groups. βER immunoreactivity was weaker in the epithelial compartment of the PIN and PC groups than in the BPH and Standard groups. CONCLUSIONS Abnormal αDG, βDG, IGFR-1, MMP-2 and MMP-9 levels certainly compromised the glandular epithelial-stromal interaction in the senescence. The differential steroid hormone receptor reactivities in the glandular lesions in both prostatic compartments indicated different paracrine signals to the dynamics of the prostate and pointed out the importance of estrogenic pathways in the activation of these changes. Also, these findings indicated that there was a direct association between IGFR-1, MMPs and steroid hormone receptors, pointing towards IGFR-1 as a target molecule in prostate therapy and a possible factor for MMPs positive signaling. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e291-e292 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Valéria Quitete Campinas, Brazil More articles by this author Amanda Hetzl Campinas, Brazil More articles by this author Wagner Fávaro Campinas, Brazil More articles by this author Ubirajara Ferreira Campinas, Brazil More articles by this author Athanase Billis Campinas, Brazil More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...