724 EVALUATION OF INFECTION AND INFLAMMATION IN THE SPINAL CORD-INJURED, NEUROGENIC BLADDER ANIMAL FOLLOWING ESCHERICHIA COLI URINARY TRACT INFECTION

Abstract

You have accessJournal of UrologyPediatrics: Basic Research1 Apr 2012724 EVALUATION OF INFECTION AND INFLAMMATION IN THE SPINAL CORD-INJURED, NEUROGENIC BLADDER ANIMAL FOLLOWING ESCHERICHIA COLI URINARY TRACT INFECTION Rajeev Chaudhry, Yuping Tang, Zarine Balsara, Sherry Ross, and Patrick Seed Rajeev ChaudhryRajeev Chaudhry Durham, NC More articles by this author , Yuping TangYuping Tang Durham, NC More articles by this author , Zarine BalsaraZarine Balsara Durham, NC More articles by this author , Sherry RossSherry Ross Durham, NC More articles by this author , and Patrick SeedPatrick Seed Durham, NC More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.809AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Recurrent urinary tract infections cause significant morbidity in patients with neurogenic bladder (NB). Our lab has established an animal model of experimental Escherichia coli urinary tract infection (UTI) in the spinal cord-injured (SCI) rat. Using this model, we have studied the natural course of acute and chronic UTI in SCI versus spinal cord-intact rats and demonstrated enhanced susceptibility to UTI in SCI rats. We now address the consequences of early antibiotic therapy on UTI in the SCI-NB animal, hypothesizing that, despite abrogation of infection, SCI-NB rats have persistent elevated inflammation of the urinary tract. METHODS Rats underwent T10 spinal cord transection or sham surgery (spinal cord-intact). After a 14 day post-operative recovery, the SCI and sham animals were inoculated with 1.2 x 104 CFU and 1.1 x 107 CFU of the human cystitis E. coli isolate UTI89, respectively. Antibiotic treatment with enrofloxacin was initiated 48 hr post-infection and continued for 5 days. Urine samples were obtained by twice daily crede and plated for enumeration of CFU. Animals were subsequently sacrificed, and bladder and kidneys were harvested and processed for enumeration of CFU, and measurement of IL-6. RESULTS UTI was established in 83% and 100% of SCI and sham rats, respectively. Bacteriuria was cleared from all animals by 24 hr of antibiotic treatment. E. coli was recovered from bladder homogenates from 33% of SCI rats, indicating persistent bacterial reservoirs despite clearance of bacteriuria. No bacteria were isolated from kidney homogenates in any animals. Mean IL-6 levels in organ homogenates from SCI rats and sham rats after 5 days of antibiotics were 7.62 ± 1.71 vs. 4.23 ± 3.51 pg/g of bladder tissue, respectively (p=0.36), and 55.38 ± 10.50 vs. 14.58 ± 3.675 pg/g of kidney tissue, respectively (p=0.04). IL-6 levels were greater in the kidneys vs. bladders in SCI (p=<0.01), but not sham animals (p=0.2). CONCLUSIONS Despite early control and clearance of bacteriuria in experimental E. coli UTI, SCI animals have evidence of elevated IL-6 in kidneys as compared to sham animals. This may suggest persistence of inflammation in the upper urinary tract that may lead to chronic inflammatory changes. Future studies will address the consequences of chronic inflammation and recurrent infections. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e297 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information Rajeev Chaudhry Durham, NC More articles by this author Yuping Tang Durham, NC More articles by this author Zarine Balsara Durham, NC More articles by this author Sherry Ross Durham, NC More articles by this author Patrick Seed Durham, NC More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...