Abstract
Fractionated bone marrow cells from normal volunteers can regularly be induced to bear the human T-lymphocyte antigen (HTLA) marker to form E-rosettes and respond to concanavalin A (Con A) following coculture with normal thymic epithelial mono-layers (TEM) or supernatants (SUP). T-cell differentiation done in this way on marrow cells of 5 patients with SCID revealed the following results: Previously, Touraine et al found no induction of HTLA marker in the marrow of a patient with SCID after incubation with thymic extract suggesting absence of stem cells but Incefy et al showed that some patients may have stem cells inducible to HTLA but not to E-rosettes or functional markers. We conclude that in variants of SCID precursor cells are present but are arrested at different steps along the T-cell line. In T-cell differentiation HTLA marker probably appears before E-rosettes, followed by a state of responsiveness to mitogens. (Supported by grants CA-17404, CA-19267 and Judith Harris Selig Foundation)
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