Abstract
A direct role for heterotrimeric G proteins in signal transduction through the T cell receptor complex has been hypothesized, but never definitively demonstrated, Nonetheless, several lines of evidence support the importance of G proteins in lymphocyte activation and differentiation events. In this study, PCR was used to generate cDNA segments of murine Gα genes from a lymphocyte cell line by amplifying areas of divergent sequence between conserved primer sites. These murine cDNAs are homologous with previously reported Gαs, Gαi2, and Gαi3 genes from other tissues and were used as probes to determine their levels of mRNA expression in a mitogen- and antigen-stimulated T lymphocyte line, 71.67, which provides a model for T cell activation. mRNA levels for all three Gα genes were found to be differentially down-regulated with 71.67 activation, but not with stimulation of three more immature lymphocyte cell lines. These findings suggest that an important element in signal transduction through these G proteins in lymphocytes may include regulation of Gα mRNA steady-state levels.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
