Abstract
THE numerous 70S ribosomes found in bacterial extracts are believed to arise from the degradation of polysomes during preparation of the extracts. But do 70S ribosomes exist at all in vivo and, if so, to what extent ? Mangiarotti and Schlessinger1 reported that in extracts of fragile mutants of E. coli 70S ribosomes always comprised less than 2 per cent of the total ribosome population. Kohler, Ron and Davis2, however, have shown that the amount of 70S ribosomes in Mangiarotti and Schlessinger's preparations was artefactually low.
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