703 CIRCULATING TUMOR CELLS CAN BE DETECTED IN PATIENTS WITH TESTICULAR GERM CELL TUMORS

Abstract

You have accessJournal of UrologyPenis/Testis/Urethra: Benign & Malignant Disaese (I)1 Apr 2013703 CIRCULATING TUMOR CELLS CAN BE DETECTED IN PATIENTS WITH TESTICULAR GERM CELL TUMORS Christian Ruf, Paulina Nastaly, Pascal Becker, Hendrik Isbarn, Friedemann Honecker, Klaus Pantel, Sabine Riethdorf, Dirk Hoeppner, Margit Fisch, Walter Wagner, and Sascha Ahyai Christian RufChristian Ruf Hamburg, Germany More articles by this author , Paulina NastalyPaulina Nastaly Hamburg, Germany More articles by this author , Pascal BeckerPascal Becker Hamburg, Germany More articles by this author , Hendrik IsbarnHendrik Isbarn Hamburg, Germany More articles by this author , Friedemann HoneckerFriedemann Honecker Hamburg, Germany More articles by this author , Klaus PantelKlaus Pantel Hamburg, Germany More articles by this author , Sabine RiethdorfSabine Riethdorf Hamburg, Germany More articles by this author , Dirk HoeppnerDirk Hoeppner Berlin, Germany More articles by this author , Margit FischMargit Fisch Hamburg, Germany More articles by this author , Walter WagnerWalter Wagner Hamburg, Germany More articles by this author , and Sascha AhyaiSascha Ahyai Hamburg, Germany More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.261AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The available serum tumor markers (AFP, β-HCG, LDH) are increased in only approximately 60% of testicular germ cell tumors (TGCT) patients. Therefore, additional markers would facilitate clinical diagnosis, staging and treatment of these patients. Detection of circulating tumor cells (CTC) has been associated with poor prognosis in other malignancies (e.g. Prostate-, Colon- and Breastcancer), however, little is known about the presence of CTC in blood of TGCT patients. METHODS In order to select the most appropriate markers for CTCs detection, the expression of different epithelial and germ cell-specific markers was examined in 4 different TGCT cell lines (TCam-2/2102Ep/NCCIT/NT2) as well as 12 histologically different primary testicular tumors. Peripheral and testicular vein blood samples from 73 and 12 patients, respectively, were collected and examined for CTCs. All samples were enriched for mononuclear cells using Ficoll density gradient centrifugation and CTCs were detected by alkaline phosphatase (AP) enzymatic activity and immunocytochemistry using anti-keratin, anti-EpCAM and anti-SALL4-antibodies. Additionally, peripheral blood samples were tested for the presence of CTCs by the automated CellSearch® system. Patients with ≥1 CTC/sample were classified as CTC-positive. To compare results for testicular and peripheral blood, CTC yields were calculated as number of CTC per 1 ml of blood. RESULTS Based on the previous analyses, double immunofluorescent staining for SALL4 and keratin, as well as analysis of AP activity were performed to detect CTCs. According to both detection systems û the one based on selected markers and automated CellSearch®, 7 (58.3%) testicular vein and 13 (17.8%) peripheral blood samples showed presence of at least one CTC. The CTC number ranged from 5 to 108/ml and 0.13 to 2.18/ml of testicular vein and peripheral blood, respectively. CTC were detected in seminoma and non-seminoma, in clinically metastasized and non-metastasized stages. CONCLUSIONS This is the first study to demonstrate CTC detection in TGCT patients. The proposed detection systems seem to be specific and sensitive for the identification of CTC. An association between CTC and histological subtype as well as metastatic stage is under investigation and will be presented at the meeting. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e289 Peer Review Report Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Christian Ruf Hamburg, Germany More articles by this author Paulina Nastaly Hamburg, Germany More articles by this author Pascal Becker Hamburg, Germany More articles by this author Hendrik Isbarn Hamburg, Germany More articles by this author Friedemann Honecker Hamburg, Germany More articles by this author Klaus Pantel Hamburg, Germany More articles by this author Sabine Riethdorf Hamburg, Germany More articles by this author Dirk Hoeppner Berlin, Germany More articles by this author Margit Fisch Hamburg, Germany More articles by this author Walter Wagner Hamburg, Germany More articles by this author Sascha Ahyai Hamburg, Germany More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...