Abstract
Publisher Summary This chapter discusses the localization and assay of guanylate cyclase. Vertebrate retinal rod outer segments contain high levels of guanylate cyclase activity. Its localization in the outer segment has been demonstrated by two sorts of experiment. After retinal homogenates have been subjected to isopycnic centrifugation in continuous sucrose density gradients, the distribution of guanylate cyclase activity approximately parallels the distribution of rhodopsin. The guanylate cyclase found in almost all other tissues either is soluble or can be solubilized by nonionic detergents. However, attempts to solubilize the guanylate cyclase of retinal rod outer segments have met with very limited success. While developing a procedure for isolating axonemes from bovine retinal rods, it is observed that guanylate cyclase copurifies with structures that include rod axonemes, basal bodies, and centrioles.
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