Abstract
Publisher Summary This chapter discusses the uses of transposons with emphasis on Tn 10. As transposable elements have become indispensable tools for bacterial genetics, many different types of specialized transposon derivatives have been constructed. The most widely used constructs are derived from insertion sequence (IS)-based elements (Tn10 and Tn5) or from bacteriophage Mu; constructs based on cointegrate-forming elements are also available. This chapter discusses the major types of transposon constructs available and describes a method to best choose the most appropriate construct for the desired application. The chapter also discusses methods used for Tnl0-derived transposon vehicles and the most recent set of useful Tnl0 vehicles..
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