7] - Three-Dimensional Computer Reconstruction of Perforated Synapses

Abstract

Publisher Summary This chapter provides an overview of the three-dimensional (3D) computer reconstruction of perforated synapses (PSs) in the neocortex as synaptic type has been implicated in the dynamic changes of synaptic plasticity in the post-developmental central nervous system. Particles that undergo structural modification are particularly suitable for 3D reconstruction because dynamic morphological changes can be clearly appreciated. It is for this reason that, for the study of PSs in the neocortex, the application of 3D reconstruction procedures is preferred. It is a naturally occurring process in which synaptic connections are remodeled and/or replaced in response to various environmental stimuli. Computer-assisted, 3D reconstruction methods for highlighting qualitative age-related differences among groups of synapses have considerable potential. Some of their morphological features, such as profile area, terminal volume, and post-synaptic density (PSD) complexity, can be quantified for statistical analyses; reconstructions can be manipulated readily by altering various transformation options to produce the best reconstruction of the original synapse and can be stored for later retrieval and analysis.