Abstract
The present study is undertaken to determine if ethanol affects 7-hydroperoxycholesterol or oxysterols in rat skeletal muscle after chronic ethanol feeding. Wistar rats were fed a liquid diet containing ethanol as 35% of total calories. After 6 weeks, soleus (Type I fibre-predominant) and plantaris (Type II fibre-predominant) skeletal muscles were dissected out. We measured 7α- and 7β-hydroperoxycholest-5-en-3β-ol (7α-OOH and 7β-OOH) as well as 7α- and 7β-hydroxycholesterol (7α-OH and 7β-OH) and 3β-hydroxycholest-5-en-7-one (7-keto). We found that in response to chronic alcohol feeding, there were significant increases in soleus 7α-OH ( P=0.0005), 7β-OH ( P=0.0005) and 7-keto ( P=0.0007), but in the plantaris, 7β-OH increased ( P=0.0418). Their elevation in chronic experimental alcoholism, together with increases in cholesterol hydroperoxides, may possibly represent evidence of increased oxidative stress.
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