Abstract
Publisher Summary Exon trapping is a widely used gene identification method. In contrast to RNA-based methods, such as screening of cDNA libraries and cDNA selection, exon trapping is independent of gene expression. Exon trapping provides a functional assay in which cloned DNA sequences are selectively isolated based on the presence of functional splice sites. Consequently, sequences are isolated directly from the clone under analysis without knowledge or availability of tissues expressing the unknown gene. This chapter discusses cosmid based exon trapping that was developed to bypass problems related to plasmid-based exon trapping. It discusses the principle of cosmid-based exon trapping. The chapter also discusses reverse transcription (RT) and amplification by polymerase chain reaction (PCR), which is the most critical step of the entire procedure.
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