6-SHOGAOL RICH GINGER OLEORESIN LOADED MIXED MICELLES ENHANCES IN VITRO CYTOTOXICITY ON MCF-7 CELLS AND IN VIVO ANTICANCER ACTIVITY AGAINST DAL CELLS

Kiran Kemkar,Kakasaheb Mahadik,Sathiyanarayanan L,Arulmozhi Sathiyanarayanan

doi:10.22159/ijpps.2018v10i1.23077

Kiran Kemkar, Kakasaheb Mahadik + Show 2 more

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https://doi.org/10.22159/ijpps.2018v10i1.23077

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Abstract

Objective: Ginger oleoresin (GO) plays an important role on the attenuation of complications associated to the cancer which is attributed to 6-shogaol (6-SGL). The major challenge in using 6-SGL for therapeutic applications is its poor aqueous solubility, low stability in GI and low bioavailability. Considering the potent anticancer nature of 6-SGL and its synergistic activity with other constituents in GO, there is a need to develop a suitable drug delivery system.Methods: Thus in the present study, 6-SGL rich GO (6-SRGO) was incorporated into mixed micelles using phospholipid (Soya Lecithin) as a carrier. The prepared 6-SRGO loaded mixed micelles (6-SRGO-LMM) were characterized physically and chemically using Fourier transform infrared spectroscopy (FTIR), Differential scanning calorimetry (DSC) and further evaluated for stability study, in vitro release study, in vitro cytotoxicity study and in vivo anticancer activity in comparison with 6-SRGO.Results: The composition such as, drug content (86.27±1.56), encapsulation efficiency (81.55±1.05) and particle size (356.11±4.07) were optimized using 32 factorial design. FTIR and DSC study confirm that the 6-SGL from 6-SRGO was entrapped in the core of phospholipid by self-assembly method to form mixed micelles. The 6-SRGO-LMM exhibited significant in vitro (GI50-23.2 μg/ml) and in vivo anticancer activity in comparison with 6-SRGO.Conclusion: We have developed and investigated mixed micelles composed of phospholipids (soya lecithin S80) and SCH as an effective nanocarrier for the delivery of a natural lipophilic anticancer bioactive 6-SGL from 6-SRGO.

Highlights

A day’s natural dietary foods including vegetables, fruits, and spices aliments have been achieved a great attraction from the scientist of various healthcare departments, food industries and the normal public because of their safety, efficacy and availability at the comparatively lower price
We have developed and investigated mixed micelles composed of phospholipids and sodium cholate hydrated (SCH) as an effective nanocarrier for the delivery of a natural lipophilic anticancer bioactive 6-SGL from 6-SGL rich GO (6-SRGO) to enhance both in vitro cytotoxicity and in vivo anticancer activity of 6-SGL
6-SRGO-loaded mixed micelles (LMM) was produced and the effect of these prepared mixed micelles composition on Drug Content (DC), EE and Particle Size (PS) was studied by 32 factorial designs [34]

Summary

Introduction

A day’s natural dietary foods including vegetables, fruits, and spices aliments have been achieved a great attraction from the scientist of various healthcare departments, food industries and the normal public because of their safety, efficacy and availability at the comparatively lower price. 6-shogaol rich ginger oleoresin (6-SRGO) is an oleoresin isolated from Zingiber officinale Rosc., consisting of higher content of phenolic ketones; gingerols and shogaols [4,5,6]. They are used for the treatment of a cough, stomach upset, inflammation, hair diseases [7], diarrhoea, nausea and vomiting. The metabolites of 6-SGL are known to possess anticancer activity [2, 13]

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