Abstract
6-Isothiocyanatobenzo [ g] phthalazine-1,4 (2 H,3 H)-dione was synthesized as a highly sensitive and selective chemiluminescence derivatization reagent for primary and secondary amines in liquid chromatography. Di- n-butylamine, dibenzylamine and n-nonylamine were used as model compounds to optimize the derivatization conditions. The reagent reacts selectively with amines in the presence of triethylamine to give highly chemiluminescent derivatives which produce chemiluminescence by reaction with hydrogen peroxide in the presence of potassium hexacyanoferrate (III) in alkaline solution. The chemiluminescent derivatives of the three amines can be separated within 12 min by reversed-phase liquid chromatography with isocratic elution, followed by chemiluminescence detection. The detection limits ( signal- to- noise ratio= 3) for primary and secondary amines are in the range 30–120 and 0.8–3 fmol for a 20-μl injection, respectively.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
