Abstract
Ethylene biosynthesis of Golden Delicious apple fruit at 20°C is rapidly inhibited by a controlled atmosphere of air + 20% CO2. However, in vitro ACC oxidase activity and ACC content were not significantly different between air and air + 20% CO2 treated fruit, To determine the in vivo effects of CO2 treatment, both in vivo and in vitro enzyme activity essays were performed in en atmosphere of air or air + 20% CO2. Western blots were also performed to quantify the amount of ACC oxidase protein present in the air and air + 20% CO2 treated fruit. We believe that in vivo cytosolic pH changes, induced by CO2, may reduce the in vivo catalytic capacity of ACC oxidase, end hence significantly reduce ethylene biosynthesis in climacteric tissue,
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