Abstract
While Random Amplified Polymorphic DNA (KAPD) has been used successfully in genetic analysis of several crop plants, the method poses difficulties with a heterozyaous species such as Theobroma cacao due to the dominant phenotypic expression of bands. A backcross family of the cultivars `Catoneo' and `Pound 12' was analyzed to determine the efficacy of this technique in analyzing cacao populations. A primary screen of the parents and F1 was conducted with 180 KAPD primers; of these, 39.5% either-did not amplify or did so poorly or irreproducibly, while 60.5% amplified well. Phenotypes produced by 42 primers represented possible test crosses, which can be used in linkage mapping. Genomic DNA from 50 individuals of the backcross population were then amplified with these 42 primers, which in most cases resulted in 1:1 segregation of bands. Preliminary experiments show that the Stoffel fragment of Taq DNA polymerase may provide additional markers. These results indicate that it should be possible to use RAPD bands as molecular markers to study the cacao genome.
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