68 - Toothbrushing Duration Effect on Streptococcus Mutans Adherence to FRC

Abstract

The purpose of this research was to study if there was an effect of toothbrushing duration on the adherence of S. mutans to FRC. The FRC sample (EverX Posterior, GC Corp, Japan) was first prepared using the mold in the shape of a disc with a diameter of 10 mm x 2 mm thickness. A total of 16 samples were prepared. Toothbrushing simulation using soft bristles was carried out on 16 samples prepared. The brushing was done with a simulated brushing tool with a speed of 250 rounds/minute and a weight of 200 grams drained in aquades. The durations selected for the simulation were 20, 40 and 60 min. A control group of 0 min toothbrushing was prepared. Streptococcus mutans were first grown, then cultured in liquid BHI medium, and incubated at 37 C for 24 hours. The turbidity of the bacterial suspension was standardized with McFarland standard of 0.5 which was equal to 1.5×10 CFU/mL. The FRC which was given the toothbrushing intervention was added into the BHI culture medium which contained S. mutans. It was then incubated at 37 C for 2 hours. After 2 hours, the FRC samples were removed, washed with PBS and vortexed for 1 minute. The solution containing the S. mutans was analyzed using ELISA reader at a wavelength of 540 nm. Statistical analysis of Anova and LSD were examined after obtaining the data. The results showed the optical density value mean of 0, 20, 40, and 60 minutes of toothbrushing were 0.04300.0003, 0.04310.0005, 0.04320.0012, and 0.0530.0005. Statical analysis showed that there was a significant effect of toothbrushing duration on the adherence of S. mutans on FRC (p<0.05). LSD result showed there was a significant difference between the 60 minutes group and the others (p<0.05). It can be concluded that the toothbrushing simulation duration up to 60 minutes increased the number of S.mutans adherence to the FRC sample.