Abstract
32 cloned probes from a walnut (Juglans sp.) PstI random genomic library were used to develop a linkage map for walnut. Low copy number walnut random genomic DNA probes were constructed and hybridized to restriction endonuclease digested DNA from parent walnut trees from a backcross of (J. hindsii × J. regia) with J. regia to identify parental polymorphism. 63 backcross progeny were analyzed to determine the inheritance and linkage of 48 RFLP loci. 66% of the probes detected duplicated, but unlinked loci. 42 of the RFLP loci could be placed on 12 linkage groups. The other 6 loci could not be placed on common linkage groups. (Theoretical maximum number of linkage groups is 16.) A Poisson probability method for estimating genome size was utilized to calculate the approximate walnut genome length as 1660 cm and to estimate that 138 markers would be needed to cover 95% the walnut genome within 20 cM of each marker.
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