Abstract
Purpose This study aims to evaluate the effects of combined surgery of myoblast sheets (MS) implantation and left ventricular restoration (LVR). Methods and Materials Myoblasts were isolated from skeletal muscle of 3-week-old Lewis rats. They were incubated and made into MS using temperature-responsive culture dishes. Rat myocardial infarction model was established 2 weeks after left anterior descending artery ligation. They were divided into 3 groups: sham operation (n=15; Group Sham), LVR by plicating the infracted area (n=15; Group LVR) and MS implantation with LVR (n=15; Group MS+LVR). Results Echocardiographic study revealed significant LV re-dilatation and decrease of ejection fraction 4 weeks after LVR in Group LVR. MS implantation combined with LVR prevented those later deteriorations of LV function in Group MS+LVR. Four weeks after the operation, a hemodynamic assessment using a pressure-volume loop showed significantly preserved diastolic function in Group MS+LVR; end-diastolic pressure (LVR vs. LVR+MS: 9.0 ± 6.6 vs. 2.0 ± 1.0 mmHg, p Download : Download high-res image (57KB) Download : Download full-size image Conclusions MS implantation decreased cardiac fibrosis by suppressing the pro-fibrotic gene expression, and attenuated the impairment of diastolic function and the late remodeling after LVR.
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