Abstract
Studies of benzyl adenine (BA) uptake and metabolism in Petunia leaf explants during shoot organogenesis revealed a novel BA conjugate. The new conjugate comprised up to 39% of the total pool of BA conjugates in two petunia lines and was associated with an increased shoot organogenic response when compared with a third petunia line which did not produce the conjugate. To identify the new compound, structural analysis was conducted using FAB mass spectrometry, two methods of carbohydrate analysis, UV absorption spectra and FTIR spectra. Experimental results identified the compound as 6-benzylamino-9-[O-glucopyranosyl-(1→3)-ribofuranosyl]-purine, a disaccharide conjugate of BA. Based on biological studies and similarity to other cytokinin conjugates, this disaccharide cytokinin conjugate may be part of the interconvertible pool of cytokinins active in shoot organogenesis.
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