Abstract
NR1 is an essential subunit of the NMDA receptor which, at the spinal level, is involved in injury-induced pain hypersensitivity and morphine tolerance. An in vitro luciferase assay was used to identify candidate and control (inactive) siRNA sequences that are expressed by a recombinant adeno-associated virus (rAAV) plasmid. rAAV vectors targeting the NR1 subunit were prepared that express active or control (mismatch) siRNA sequences and injected into the mouse spinal cord dorsal horn (SCDH). Three weeks after vector administration, GFP labeling of the ipsilateral SCDH confirmed the spatial localization of the viral transduction.
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