Abstract
The purpose of this study was to observe the difference in leaf surface characteristics between in vitro (vitrified and glaucus) and greenhouse grown onion leaves. Scanning electron microscopy (SEM) was used. Vitrified (hyperhydrated) leaves had little epicuticular wax and the leaf surface appeared twisted and wavy. Stomata were raised above the epidermis, appeared round instead of oblong and openings were malformed and clogged with waxy deposits. Both glaucus in vitro and greenhouse leaves had thick layers of epicuticular wax, the wax appearing to be heavier on greenhouse leaves. Stomata were oblong and sunken beneath layers of epicuticular wax. Vitrified plants died when transferred to the greenhouse under mist, while glaucus in vitro plants survived transplanting. Vitrified plantlets have a translucent, glass-like appearance and do not survive transfer to ex vitro conditions. Factors affecting vitrification will be addressed in later publications.
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