Abstract
For studies of the regulation of protein synthesis in higher organisms and for the isolation of specific mRNA's, it is necessary to have sensitive and quantitative assays of mRNA content of tissues. This chapter found the crude rabbit reticulocyte lysate system to be an adequate system for such assays. The techniques are simple, and specific protein synthesis directed by exogenous RNA can be monitored readily by use of highly specific immunologic techniques. Although the procedures described in the chapter are for the synthesis of ovalbumin, these techniques work equally well for conalbumin and rat serum albumin mRNA's. Thus the reticulocyte lysate system may provide a general method for the quantitative measure of mRNA's from animal tissue.
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