618-P: Effect of Liraglutide Combined with Human Umbilical Cord Mesenchymal Stem Cells on Liver Injury of T2DM with NAFLD Rats by Mediating Toll-Like Receptor 4/Nuclear Factor-kappa B Signaling Pathway and Oxidative Stress

Abstract

Objective: To observe the effect of liraglutide combined with human umbilical cord mesenchymal stem cells on type 2 diabetes mellitus with nonalcoholic fatty liver disease rats liver injury. And then to investigate relation of the effect on the liver injury between the toll-like receptor 4/nuclear factor-kappa B inflammatory signal pathway and oxidative stress mechanism. Methods: Animal model establishment and experimental grouping and intervention: The rats were randomly selected and divided into the following four groups: T2DM with NAFLD model group, hUC-MSCs treatment group, Liraglutide treatment group, Liraglutide combined with hUC-MSCs treatment group. Results: 1. Rat serum levels of TNF-α, IL-6, 8-OHdG and SOD: Compared with MC+LIRA group, the levels of TNF-α, IL-6 and 8-OHdG were decreased in MC+LIRA+hUC-MSCs group (P <0.05), SOD was increased (P<0.05). 2. Pathological changes of rats liver tissue:Compared with MC+LIRA group, the NAS score were decreased in MC+LIRA+hUC-MSCs group (P <0.05).3. Expression of TLR4 and NF-κB mRNA in rat liver tissue: Compared with MC+hUC-MSCs group, the expression of TLR4 and NF-κB mRNA were significantly decreased in MC+LIRA group and MC+LIRA+hUC-MSCs group (P<0.05). Compared with MC+LIRA group, the expression of TLR4 and NF-κB mRNA were decreased in MC+LIRA+hUC-MSCs group (P <0.05). Conclusion: The mechanism of liraglutide combined with human umbilical cord mesenchymal stem cells on type 2 diabetes mellitus with nonalcoholic fatty liver disease is closely related to the down-regulation of TLR4/NF-κB inflammation pathway and oxidative stress. Disclosure P. Chen: None.