Abstract
The effect of node position of axillary buds of greenhouse-grown Hybrid tea rose cultivars `Carefree' and `Adelaide Hoodless' on shoot induction and proliferation was investigated. Explants were cultured on a Murashige and Skoog (MS) medium containing 2 mg/l 6-benzyladenine (BA) and 0.1 mg/l α-napthaleneacetic acid (NAA). Axillary buds collected closest to the shoot apex gave rise to the highest shoot proliferation; whereas, those collected from the second, third, and fourth nodes yielded longer shoots and higher leaf number/explant. The influence of varying levels of BA concentrations in the culture medium was also investigated. It was observed that high BA concentrations, 1-3 mg/l, stimulated shoot proliferation. The effect of carbohydrate source, fructose versus surcose, on shoot induction was also investigated. A high shoot number/explant was observed on media containing fructose; however, longer shoots (>1 cm) were observed on media containing surcose as a source of carbon.
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