617 - WNT 5A Expression Has Assocation with VEGFR and MMP-9 Expression in Primary or Metastatic Tumor Tissue in Colorectal Cancer

Abstract

ABSTRACT Background It has been known that various molecular changes can develop as cancer progresses. However, it is difficult to confirm the progressive change in the human tissue because adequate tumor tissue cannot be obtained from metastatic site. Resection for metastatic lesion is commonly done in metastatic colorectal cancer in spite of palliative surgery. The Wnt, VEGFR, and MMP protein expression have been explored in advanced cancer due to its association with invasion and metastasis. We investigated these protein expressions in primary tumor and metastatic site to get the basic information of the role of these proteins in cancer progression. Method Tissue specimens from 130 patients with metastatic colorectal cancer were analyzed. All patients took resection for primary tumor and metastatic lesion between Jan, 2000 and Aug, 2008 at Seoul St. Mary's hospital, the Catholic University. We performed immunohistochemical staining using tissue microarray from the primary tumor tissue and metastatic site for Wnt 3a, Wnt 5a, VEGFR, and MMP-9. Result Of the 100 patients, Male was 59, and female was 41. Colon cancer was 60 and rectal cancer was 40. In primary tumor, Wnt 3 & 5 expression was 72% and 70% respectively. VEGFR expression was 24% and MMP-9 was 39%. In metastatic site, Wnt 3 & 5 expression rate were slightly decreased to 66%. However, VEGFR expression rate was slightly increased to 30% and MMP-9 expression rate was same. In concordance rate between primary and metastatic site, Wnt 3a expression showed 68% and 62% in Wnt 5a, 66% in VEGFR, 68% in MMP-9. There was no significant change of expression between primary and metastatic site. There was no association between Wnt 3a expression and VEGFR or MMP-9 expression both in primary tumor and metastatic site. However, Wnt 5a expression showed significantly correlation with negative VEGFR expression (p = 0.02 both in primary tumor and metastatic site. Wnt 5a expression was also associated with negative MMP-9 expression in primary tumor tissue (p = 0.022). Conclusion We could not find any significant change in protein expression between primary and metastatic site. However, there was a tendency of more VEGFR expression in metastatic site than primary tumor. The Wnt 5 expression was associated significantly negative expression of VEGFR and MMP-9. Disclosure All authors have declared no conflicts of interest.