Abstract
Objective To determine the clinical significance of α6β1 integrin in human spermatozoa. Design Prospective case-controlled study. Setting Hospital infertility clinic in India. Patient(s) Sixty-two men undergoing evaluation for infertility and 20 healthy, proven-fertile controls were selected. Intervention(s) Role of α6β1integrin in sperm quality evaluation was determined using semen analysis, cell ELISA, flow cytometry, and in vitro sperm–egg binding. Main outcome measure(s) Determination of sperm-associated α6β1 integrin may help in assessing the quality of the human spermatozoa. Result(s) Expression of α6β1 integrin was significantly low in the spermatozoa obtained from subfertile men. Only 35%–40% of spermatozoa from fertile men showed a positive reaction to α6β1 antibody. The samples with high rate of acrosomal reaction showed a good expression of α6β1 and maximum binding to oocyte in an in vitro binding assay. Conclusion(s) α6β1 Integrin may be used as a clinical marker to evaluate sperm quality.
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