Year-end Sale % OFF 
Abstract
Postharvest germination negatively affects the development of fresh walnut industry. Our previous research showed that 60Coγ-ray irradiation with 0.3 kGy effectively inhibited the germination of fresh walnuts during storage, but the underlying mechanism in respiratory metabolism remains still unclear. The untreated and 0.3 kGy-irradiated fresh walnuts were stored at 25 °C for 24 d, and then the indicators related to respiratory metabolism in kernels and embryos were determined. The results found that the 0.3 kGy irradiation decreased the respiration intensity after 15d and completely inhibited the germination of fresh walnuts with the declined adenosine triphosphate (ATP) content and energy charge (EC) levels in kernels except from 12 to 15 d and polyphenol oxidase (PPO), cytochrome C oxidase (CCO) and ascorbic acid oxidase (AAO) activities in both tissues except the stimulated PPO activity in embryos during storage. Moreover, the irradiation promoted the glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH) activities but reduced the isocitrate lyase (ICL) activity in both tissues, and decreased the nicotinamide adenine dinucleotide reduced form (NADH) content but increased the nicotinamide adenine dinucleotide phosphate reduced form (NADPH) content in kernels of fresh walnuts during storage. The irradiation induced the JRG6PDH and JR6PGDH expression levels in both tissues, as well as the JRICL and JRMS expression levels in kernels before 12 d but suppressed those after 12 d of storage. In conclusion, the germination inhibition of fresh walnuts by gamma irradiation is attributed to suppressed terminal oxidases activities, decreased ratio of Embden–Meyerhof–Parnas pathway-tricarboxylic acid cycle (EMP-TCA) and glyoxylate cycle (GAC), increased level of pentose phosphate pathway (PPP) and reduced energy status.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
