60Co irradiation of Shiga toxin (Stx)-producing Escherichia coli induces Stx phage

Abstract

Shiga toxin (Stx)-producing Escherichia coli (STEC), an important cause of hemolytic uremic syndrome, was completely killed by 60Co irradiation at 1×l0 3 gray (1 kGy) or higher. However, a low dose of irradiation (0.1–0.3 kGy) markedly induced Stx phage from STEC. Stx production was observed in parallel to the phage induction. Inactivation of Stx phage required a higher irradiation dose than that for bacterial killing. Regarding Stx, cytotoxicity was susceptible to irradiation, but cytokine induction activity was more resistant than Stx phage. The findings suggest that (1) although 60Co irradiation is an effective means to kill the bacteria, it does induce Stx phage at a lower irradiation dose, with a risk of Stx phage transfer and emergence of new Stx-producing strains, and (2) irradiation differentially inactivates some activities of Stx.