6073 Interim analysis of epidermal-growth factor receptor (EGFR) expression in a single-arm, phase II, first-line study (20060314) of panitumumab with FOLFIRI in the management of metastatic colorectal cancer (mCRC)

Abstract

Aerobic yeast mitochondria contain a unique isozyme of acetaldehyde dehydrogenase that accounts for the ability of yeast mitochondria to respire with acetaldehyde as a substrate. Mitochondrial acetaldehyde dehydrogenase has a high affinity for acetaldehyde, is active with NAD+ and NADP+, is stimulated by K+, and appears to be the same acetaldehyde dehydrogenase previously isolated from yeast autolysates by Black ((1951) Arch. Biochem. Biophys. 34, 86–97) and Jakoby and coworkers (Steinman, C. R. and Jakoby, W. B. (1967) J. Biol. Chem. 242, 5019–5023; Steinman, C. R. and Jakoby, W. B. (1968) J. Biol. Chem. 243, 730–734; Clark, J. F. and Jakoby, W. B. (1970) J. Biol. Chem. 245, 6065–6071; Clark, J. F. and Jakoby, W. B. (1970) J. Biol. Chem. 245, 6072–6077; Bradbury, S. L. and Jakoby, W. B. (1971) J. Biol. Chem. 246, 1834–1840; Bradbury, S. L. and Jakoby, W. B. (1972) Proc. Natl. Acad. Sci. U.S. 69, 2373–2376). The enzyme is inhibited by Li+, Mg2+, and Disulfiram. Mitochondrial acetaldehyde dehydrogenase appears to play a role in the oxidative metabolism of ethanol, especially when yeast cells are utilizing ethanol as a source of carbon and energy for growth. The enzyme is more than 99% repressed during the growth of yeast on glucose.Yeast cells also contain a cytosol isozyme of acetaldehyde dehydrogenase that is active with NADP+, is stimulated by Mg2+, and is only partly repressed during growth on glucose. The cytosol isozyme resembles the acetaldehyde dehydrogenase previously prepared from yeast autolysates by Seegmiller ((1953) J. Biol. Chem. 201, 629–637).