601 Effects of hypertension on adriamycin-induced cardiomyopathy of rats

Abstract

s S81 601 Effects of hypertension on adriamycin-induced cardiomyopathy of rats H.-J. Youn, S.-H. Ihm, C.-S. Park, E.-J. Cho, H-O. Jung, H.-K. Jeon, J.-H. Kim, S.-J. Hong, Y.-S. Choi. Catholic University of Korea, Internal Medicine, College of Medicine, Seoul, Korea, Republic of Aim: We intended to elucidate the eflects ol hypertension on adriamycin-induced cardiomyopathy. Methods: Normotensive Wislar-Kyolo rats (WKY) and spontaneously hypertensive rats (SHR) were randomly divided into adriamycin-lrealed groups or salinetreated groups. Adriamycin with the dosage ol 2.5 mg/kg was administrated intraperitoneally ol 6 equal injections lor 3 weeks for adriamycin-lrealed groups, and normal saline with same volume was administrated lor saline-treated groups. Translhoracic echocardiography with a 15 MHz linear array transducer was performed at baseline and at sixth week to measure lractional shortening ol the lell ventricle. Histologic analyses used picrosirius red stain were performed for quantifying fibrosis. Results: (1) Adriamycin4reated WKY group, the lelt ventricular systolic lunction was decreased significantly (P=0.002), and fibrosis ol perivascular and interstitial area ol the myocardium was increased significantly (P=0.001, P=0.002), compared with saline4realed WKY group. (2) Saline-treated SHR group, fibrosis ol perivascular and interstitial area ol the myocardium was increased significantly, compared with saline4reated WKY group (P=0.002, P=0.002). (3) Adriamycin4realed SHR group, the fibrosis on perivascular area ol the myocardium was increased significantly, compared with saline4realed SHR group and adriamycin-lrealed WKY (P=0.001, P=0.001). Conclusion: These results suggest the hypertension increases the perivascular and interstitial fibrosis, resulting in aggravation ol adriamycin-induced cardiomy-