600 CHANGE OF HUR EXPRESSION BY INTAKE OF GREEN TEA POLYPHENOL IN CHEMICAL INDUCED BLADDER CANCER ANIMAL MODEL

Abstract

You have accessJournal of UrologyBladder Cancer: Basic Research (I)1 Apr 2013600 CHANGE OF HUR EXPRESSION BY INTAKE OF GREEN TEA POLYPHENOL IN CHEMICAL INDUCED BLADDER CANCER ANIMAL MODEL Yasuyoshi Miyata, Kensuke Mitsunari, Tomohiro Matsuo, Kojiro Ohba, and Hideki Sakai Yasuyoshi MiyataYasuyoshi Miyata Nagasaki, Japan More articles by this author , Kensuke MitsunariKensuke Mitsunari Nagasaki, Japan More articles by this author , Tomohiro MatsuoTomohiro Matsuo Nagasaki, Japan More articles by this author , Kojiro OhbaKojiro Ohba Nagasaki, Japan More articles by this author , and Hideki SakaiHideki Sakai Nagasaki, Japan More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.1996AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Human antigen R (HuR) is a member of RNA-binding proteins, and is reported to regulate the stability of m-RNA. HuR plays important roles in carcinogenesis and tumor growth via regulation of angiogenesis-related molecules in various cancers However, pathological roles of HuR in bladder cancer remains unclear. In addition, regulative mechanism of HuR expression in cancer cells is also unknown. The main purpose of this study is to clarify carcinogenic function and malignant potential of HuR in bladder cancer. In addition, we also investigated change of HuR expression by green tea polyphenol (GTP) in chemical induced bladder cancer animal model. METHODS In this study, HuR expression was evaluated separately with cytoplasmic and nuclear staining. We investigated HuR expression in 122 human bladder cancer tissues and 20 mice of chemical induced bladder cancer model that 8-week-old mice were treated with N-Butyl-N-[4-hydroxybutil] nitrosamine (BBN) solution for 14 or 24 weeks. In addition, these mice were also treated with and without 0.5% GTP solution for the same periods. HuR expression was evaluated by immunohistochemical technique and microvessel density (MVD) was estimated by counting CD34-positive vessels in the tumor area. We previously confirmed that non-muscle invasive bladder cancer (NMIBC) and MIBC was occurred by treated with BBN for 14 and 24 weeks, respectively. RESULTS In BBN group, high nuclear expression was found in 80% (8/10) and 70% (7/10) of normal urothelium and cancer cells, respectively. On the other hand, although high cytoplasmic HuR expression in normal cells was detected in 10% (1/10), its high expression in NMIBC and MIBC was detected in 40% (2/5) and 80% (4/5), respectively. In human tissues, high HuR expression was found in 14.6% (15/103) and 84.2% (16/19) of NMIBC and MIBC, respectively. In BBN+GTP group, rate of high HuR expression in NMIBC was similar (40%, 2/5) to that in BBN group; however, its rate in MIBC (40%, 2/5) was lower than that in BBN group. In regard to MVD in MIBC, median and range of MVD in BBN+GTP group (6.2 and 4.0-12.2/high power field, HPF) was significantly (P<0.01) lower than that in BBN group (12.2 and 4.7-22.2/HPF). Furthermore, MVD was correlated with HuR expression in both groups of MIBC. CONCLUSIONS HuR expression was associated with carcinogenesis and tumor growth in bladder cancer. In addition, a part of such pathological function of HuR was speculated to be regaled by angiogenesis. Interestingly, our results showed that GTP has possibility to suppress HuR expression and angiogenesis in bladder cancer tissues. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e245 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Yasuyoshi Miyata Nagasaki, Japan More articles by this author Kensuke Mitsunari Nagasaki, Japan More articles by this author Tomohiro Matsuo Nagasaki, Japan More articles by this author Kojiro Ohba Nagasaki, Japan More articles by this author Hideki Sakai Nagasaki, Japan More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...