Abstract
The effect of changing osmolality on basal and bradykinin (BK) stimulated 6-oxo-PGFIoc, PGE2, TxB2 and arachidonic acid (AA) synthesis of human skin fibroblasts was studied, In parallel, its influence on membrane fluidity was determined using 1-(4-trimethylammoniumphenyl)-6-phenyl-1, 3, 5 -hexatriene (TMA-DPH) as a marker of superficial cell membrane layers. Hypoosmolar mannitol (35 mosm/kg) onhanced basal 6-oxoPGF1α (152%), PGE2 (292%), TxB2 (181%), AA (549%) and BK stimulated 6-oxoPGF1cx (194%), PGE2 (342%), TxB2 (152%) and AA (402%) production, when compared to isotonic control (100%). rG (fluorescence anisotropy, inversely related to membrane fluidity) was significantly decreased from 0.362±0.009 to 0.281±0.024 (p < 0.001). In hyperosmolar mannitol (610 mosm/kg) the basal and BK simulated PG synthesis was either unaltered or inhibited (in BK stimulated release PGE2 was 77% and AA 70%). rG was slightly increased from 0.362±0.009 to 0.368±0.026 (p > 0.05), when compared to isotonic mannitol. Conclusions: Osmolality modifies prostaglandin production and membrane fluidity in cultured human skin fibroblasts. Since changes in osmolality result in parallel changes of PG production and membrane fluidity it might be assumed that PG production is at least in part due to changes of membrane fluidity.
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