(60)Co-.GAMMA. irradiation to establish the azoospermia male mice model

Abstract

Objective To investigate the appropriate doses and methods of 60Co-γ in establishing the azoospermia animal models. Methods 100 Kunming mice were randomly divided into groups A, B, C, D, E, F and G. In group A (n=25), the animals did not receive any intervention. The mice in group B (n=15 ) receive the intraperitoneal injection of cyclophosphamide, and the mice in experimental groups C, D, E and F (n=15 in each group) were subjected to different doses of 60Co-γ, onetime. The mice in group G (n=15) were given 2 times of 60Co-γ. Observation went on for 35 days after the model establishment. Pregnancy tests were done, testicular weight was weighed, and testis index was calculated. FSH, LH, T, and E2 levels in serum were determined by enzyme-linked immunoassay. Testicular biopsy was carried out for histological observation. Results Groups C, D and G had the lowest mortality rate, but pregnancy rate in groups F and G was reduced to zero; Testicular tissue damage caused by irradiation induced the lower T (5. 62±2. 89) and E2(53. 28 ±2. 00) level, also the irradiation brought injury to hypothala-mus and pituitary which led the changes of FSH (3. 00±1.42) and LH (30.10± 9.86) (as compared with group A); C-kit in group A was expressed in the brown ring, whereas in other groups the expression performed fragment or none. Conclusion The azoospermia animal model can be created by 60Co-γ radiating twice in an interval of 6 Gy and 4 Gy. Key words: 60Co-γ; Azoospermia; Model,animal