6-shogaol suppresses oxidative damage in L6 muscle cells

Jinyoung Hur,Chang Jun Lee,Sang Yoon Choi,Ho-Young Park,Yeonmi Lee

doi:10.1186/s13765-020-00544-8

Jinyoung Hur, Chang Jun Lee + Show 3 more

Open Access

https://doi.org/10.1186/s13765-020-00544-8

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Abstract

Ginger (Zingiber Officinale Roscoe) has been known reduce muscle pain after exercise, and 6-shogaol {(E)-1-(4-Hydroxy-3-methoxyphenyl)dec-4-en-3-one)} is the major essential oil contained in ginger. In this study, the protective effect of 6-shogaol on L6 muscle cells against oxidative damage was measured. 6-shagol inhibited the damage of L6 cell induced by H2O2, and allowed the increase in mRNA and protein expression levels of intracellular HO-1 and NRF2. 6-shogaol also reduced the production of intracellular ROS. These results suggested that 6-shagol effectively inhibits oxidative damage of skeletal muscle cell.

Highlights

Active oxygen is produced in the normal metabolic process and it performs various biological functions; but active oxygen that is excessively generated causes damage to cells and tissues [1,2,3]
Protective effects on L6 muscle cells Violent exercise generates excessive active oxygen, and as a result, the muscle tissues are subjected to oxidative damage [18]
After confirming that L6 cell death was not induced by 100 μM of 6-gingerol, 8-gingerol, 10-gingerol, and 6-shogaol, L6 cells were treated for 24 h with 1, 10, and 100 μM of these materials along with 2 mM of hydrogen peroxide. 76.4% of the cells died when treated with only hydrogen peroxide, but when the cells were simultaneously treated with 6-shogaol and hydrogen peroxide, cell death inhibition

Summary

Introduction

Active oxygen is produced in the normal metabolic process and it performs various biological functions; but active oxygen that is excessively generated causes damage to cells and tissues [1,2,3]. Strenuous exercise causes generation of excessive active oxygen and oxidative damage in muscle tissue [6, 7]. The human body protects itself by generating antioxidant enzymes as a defense system against such oxidative damage but the protection ability varies depending on the persons’ age and health status. It has been reported that if ingesting ginger reduces post-exercise muscle pain [8]. Anti-inflammatory, and the protective effect on the cranial nerve [10,11,12,13,14] and melanocytes [15], but studies of the effect on skeletal muscle have not been

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