Abstract

Laser-assisted bioprinting (LAB) is an emerging tool for tissue engineering (TE) that allows printing of cells and liquid materials with a cell-level resolution, to reproduce the anisotropy of living tissues. We have integrated LAB in a dedicated workstation to allow rapid prototyping applications. After introducing the rationale of LAB in TE, we present the physical parameters related to laser-induced forward transfer technique, implemented in LAB. These parameters must be tuned to print viable cell patterns with respect to cell-level histological organization and to high throughput manufacturing. Finally, we present some typical multicomponent printing, 3-D printing approaches, in vitro, and in vivo.

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