Abstract
Radiation hybrid (RH) mapping is the most efficient method for generating long-range genomic maps using both polymorphic and nonpolymorphic markers. The basic principle of RH mapping is that the closer two loci are on a chromosome, the less likely they are to be separated by a radiation-induced break. Therefore, markers that are closely linked show correlated coretention patterns across the hybrid panel, where markers located at a large distance from one another are retained almost independently. There are a number of advantages to radiation hybrid mapping. RHs offer high map resolution for relatively small numbers of hybrids. The additional strength of this technique lies in allowing the efficient integration of physical and genetic maps, by facilitating the resolution of cosegregating genetic markers lying in recombination cool-spots on the meiotic map, and anchoring Yeast Artificial Chromosome (YAC) contigs to the genome map. By using a whole genome radiation hybrid (WG-RH) panel, anonymous markers can be both assigned to a chromosome and localized on that chromosome. The two most extensively used RH mapping programs are RHMAP and RHMAPPER. This chapter demonstrates the analysis of two WG-RH datasets using these programs, discussing the merits and limitations of both the packages.
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