Abstract
Publisher Summary This chapter describes the procedures for preparation and characterization of one type of versatile and stable liposomes as well as for covalent conjugation of neoglycoproteins and lectins to the liposomes, and characterization of the conjugates. To analyze the homogeneity and stability of liposomes, size distribution is an important characteristic among several physical properties. Gel-permeation chromatography (GPC), electron microscopy (EM), and dynamic light scattering (DLS) can be applied for size characterization of liposomes. The chapter discusses the procedure for GPC analysis. A GPC result is compared with analyses by EM and DLS. To analyze the purity and stability of neoglycoprotein-coupled liposomes or lectin-coupled liposomes, GPC analysis is routinely carried out by using the high-performance liquid chromatography (HPLC) system. To analyze the yield and quality of neoglycoprotein-coupled liposomes or lectin-coupled liposomes, protein and lipid compositions in each preparation are routinely determined, and coupled protein/total lipid ratios calculated. The protein content of neoglycoprotein-liposome and lectin-liposome conjugates is estimated by either employing the modified Lowry method in the presence of 1% sodium dodecyl sulfate, or by using a commercial micro-bovine serum albumin (BSA) protein assay kit in the presence of 1% sodium dodecyl sulfate.
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