Abstract
This chapter describes the application of high-pH anion-exchange chromatography, with pulsed amperometric detection (HPAEC-PAD) to the identification and partial characterization of glycopeptides, within a reversed-phase high-performance liquid chromatography (HPLC) peptide map. HPAEC is a versatile chromatographic method that can be used for the detection and quantitation of monosaccharides, released by acid hydrolysis and for the analysis of intact oligosaccharides released enzymatically or chemically. A large number of other analytical methods, including mass spectrometry (MS), enzymatic microsequencing, nuclear magnetic resonance (NMR), and capillary electrophoresis, can also be applied to the characterization of glycopeptides; in general, a combination of several complementary analytical methods are needed to achieve a definitive carbohydrate structure determination. This chapter discusses the type of information that can be obtained, using a single, comparatively simple, analytical method.
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