Abstract

3H-digitoxigenin was metabolized in vitro by rabbit liver homogenates. The major metabolite resulting from this biotransformation was isolated and identified as 6β- hydroxy-3-epidigitoxigenin. Approximately 50 per cent of the substrate was converted to this metabolite after 1 hr of incubation. The previously identified 5β-hydroxydigitoxigenin. 3-dehydrodigitoxigenin and 3-epidigitoxigenin were also present as metabolites. This is the first confirmation of the hydroxylation of digitoxigenin at C 6.

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