Abstract
A highly sensitive Surface Plasmon Resonance (SPR) sensor coupled magnetic molecularly imprinted polymers nanoparticles (MMIPs NPs) was developed and validated for the determination of 6-benzylaminopurine (6-BA) in vegetables. MMIPs NPs were synthesized using methacrylic acid (MAA) and sodium p-styrene sulfonate (SSS) as functional monomers. The SPR exhibited a linear dependence on 6-BA concentration in the range 5–300 pg/mL with a low limit of detection (3.02 pg/mL) and limit of quantitation (10.08 pg/mL). The SPR signal of 6-BA-captured MAA/SSS-MMIPs NPs is higher than those of the structural analogues (6-KT and 2-IP: 1.72 and 2.12 times) and the non-structural analogues (2, 4-D and NAA: 2.31 and 2.57 times), indicating the SPR sensor has good selectivity for 6-BA. The recovery of the established method was between 93.8% and 108.6% with a coefficient of variation less than 9.2% in four vegetables. This SPR sensor shows great potential in detecting 6-BA in more vegetables.
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