不同浓度5-氨基乙酰丙酸(ALA)浸种对NaCl胁迫下番茄种子发芽率及芽苗生长的影响

Abstract

In order to explore the effects of exogenous 5-aminolevulinic acid(ALA) on seeds germination and seedlings growth under NaCl stress,this experiment was conducted by presoaking the tomato seeds(Solanum lycopersicum cv.Zhongza9) with different concentrations of ALA(0,0.1,0.5,1.0,5.0,10.0mg/L) for 24 hours,and training under 0,25,50,and 100 mmol/L NaCl stress in growth cabinet at 28℃ for 7 days in darkness.The germination rate(GR),germination energy(GE),germination index(GI),vigor index(VI),shoot fresh weight(SFW),root fresh weight(RFW) and antioxidant enzymes(SOD,POD,CAT)activity and malondialdehyde(MDA) content in shoots and roots were emphatically analyzed in this paper.The results showed that: GE,GI,VI,FW of tomato seeds germinated with distilled water are significantly increased in ALA soaking treatment than that in non-ALA treatment,while SOD and POD activity in roots are largely reduced and MDA content decreased.Low concentration of NaCl(25mmol/L) treatment can improve GR,VI and FW,promote tomato seeds germination;high concentration of NaCl(50—100mmol/L) treatment reduce GR,GE,GI and VI,extremely inhibit seed germination;appropriate concentration of ALA(0.1—0.5mg/L) presoaking seeds can improve the GR,GI,VI,FW and antioxidant enzymes activity of tomato seeds,make MDA content decreased,have promoted effects on tomato seeds germination and after germination growth under NaCl stress;high concentration of ALA(10mg/L) presoaking seeds make GR,GI,VI reduced,inhibit seed germination.In conclusion,presoaking seeds with ALA can promote germination and after germination growth of tomato seeds,soaking concentration should not exceed 5.0mg/L,0.1mg/L ALA soaking treatment have the best effects on tomato seeds germination and after germination growth under NaCl stress.