585: Distinct vaginal microbiome profiles are associated with host mitochondrial DNA (mtDNA) haplogroup and SNP variants

Abstract

diac function in a mouse model of IUGR. STUDY DESIGN: Laparotomy was performed on pregnant C57BL/6J mice at gestational day 18 and pups were divided into 3 groups (n 12). Control: Sham operated; IUGR surgically induced by ligation of a branch of the uterine artery; IGF1Treated by intra placental injection of Ad hIGF1 after ligation. Pups were delivered on day 20, cross-fostered to CD1 mice, sorted by gender and at week 8 a western high fat diet introduced. Cross-sectional, 2D and Doppler transthoracic echocardiography was performed at 12 weeks evaluating ejection fraction (EF), fractional shortening (FS) and left ventricular (LV) dimensions. Data were analyzed using ANOVA. RESULTS: IUGR mice demonstrated significantly reduced EF (43.5 8.3 vs 60 5.1vs 59 5.7,p 0.001) and FS (22 4.6 vs 32.2 3.2 vs 31.3 3.7,p 0.001) compared to SHAM that was normalized in the treatment group (FigA). IUGR significantly increased LVID;S (3.6 0.4vs3.1 0.2vs2.9 0.2,p 0.001) as compared to SHAM, restored to normal in IGF1 Treated (FigB). These findings were the same when data was stratified by sex. CONCLUSION: Intra-placental gene transfer of Ad hIGF1 rescues IUGR induced cardiac dysfunction in mice exposed to high fat diet. This supports the concept that in utero therapy may positively impact cardiac remodeling to attenuate adult onset cardiovascular disease under environmental stress. 585 Distinct vaginal microbiome profiles are associated with host mitochondrial DNA (mtDNA) haplogroup and SNP variants Jun Ma, Kjersti Aagaard, Dirk Gevers, Aleksandar Milosavljevic, Penelope Bonnen, Curtis Huttenhouer, James Versalovic Baylor College of Medicine, Molecular and Human Genetics, Houston, TX, The Broad Institute at MIT, Genome Sequencing and Analysis, Boston, MA, Baylor College of Medicine, Pathology and Immunology, Houston, TX, Harvard, School of Public Health, Cambridge, MA, Baylor College of Medicine, Division of Maternal-Fetal Medicine, Departments of ObstetricsG SNP associations were determined wtih PLINK. RESULTS: 173 mtDNA variants (SNP/Indels) from 38 females of diverse racial/ethnic background were identified. Specific taxa (OTU) demonstrated strong association with haplogroups I, W,X and SNP T16311C, but not with other host properties (left panels). The synonymous point mutation T6776C demonstrated strong association with microbial encoded fatty acid metabolism pathways (right panels). CONCLUSION: Several Caucasion haplogroups and mtDNA variants show significant association with specific microbial taxa and metabolic pathways from the posterior fornix of the vagina. We speculate that these findings may partially explain infectious, microbiome and host associations in perinatal disorders.