Abstract
Since in vitro methods (2D and 3D) used for the preclinical testing of candidate anti-aging compounds mimic the complexity of human skin aging unsatisfactorily, we wished to develop an ex vivo assay for interrogating key aging-read outs in healthy human skin. Healthy, full-thickness skin biopsies (2 males, 4x females; 22 - 77 years) were organ-cultured in serum-free, supplemented medium for 3 days in the presence/absence of recognized anti-aging compounds (tretinoin (5μM), resveratrol (10μM), or caffeine (200μM), and key skin aging biomarkers were analyzed by quantitative (immuno-)histomorphometry and/or qRT-PCR.
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